Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.
Identifieur interne : 000383 ( Main/Exploration ); précédent : 000382; suivant : 000384Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.
Auteurs : Zhao Zhang [Pays-Bas] ; Bart P H J. ThommaSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Tabac.
- enzymologie : Tabac.
- microbiologie : Tabac.
- métabolisme : Agrobacterium tumefaciens, Oxidoreductases, Virus des plantes.
- virologie : Tabac.
- Expression des gènes, Extinction de l'expression des gènes.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Oxidoreductases.
- enzymology : Tobacco.
- growth & development : Tobacco.
- metabolism : Agrobacterium tumefaciens, Plant Viruses.
- microbiology : Tobacco.
- virology : Tobacco.
- Gene Expression, Gene Silencing.
Abstract
Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.
DOI: 10.1007/978-1-62703-986-4_14
PubMed: 24643561
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Thomma</name></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (metabolism)</term><term>Gene Expression (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Oxidoreductases (metabolism)</term><term>Plant Viruses (metabolism)</term><term>Tobacco (enzymology)</term><term>Tobacco (growth & development)</term><term>Tobacco (microbiology)</term><term>Tobacco (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (métabolisme)</term><term>Expression des gènes (MeSH)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Oxidoreductases (métabolisme)</term><term>Tabac (croissance et développement)</term><term>Tabac (enzymologie)</term><term>Tabac (microbiologie)</term><term>Tabac (virologie)</term><term>Virus des plantes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Plant Viruses</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Oxidoreductases</term><term>Virus des plantes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Gene Silencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term><term>Extinction de l'expression des gènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24643561</PMID><DateCompleted><Year>2014</Year><Month>11</Month><Day>05</Day></DateCompleted><DateRevised><Year>2014</Year><Month>03</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1127</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.</ArticleTitle><Pagination><MedlinePgn>173-81</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-62703-986-4_14</ELocationID><Abstract><AbstractText>Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomma</LastName><ForeName>Bart P H J</ForeName><Initials>BP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.99.-</RegistryNumber><NameOfSubstance UI="C022398">phytoene dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010942" MajorTopicYN="N">Plant Viruses</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>3</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>3</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24643561</ArticleId><ArticleId IdType="doi">10.1007/978-1-62703-986-4_14</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><noCountry><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name></noCountry><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Zhang, Zhao" sort="Zhang, Zhao" uniqKey="Zhang Z" first="Zhao" last="Zhang">Zhao Zhang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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